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Plasma lipids were measured using conventional enzymatic methods and total lipids were calculated as described [ 31].
Serum lipid and glucose levels were determined using conventional enzymatic methods.
Total cholesterol, HDL cholesterol, and triglyceride concentrations were measured using conventional enzymatic methods, with LDL cholesterol calculated using the Friedewald formula (32).
Serum lipids were measured using conventional enzymatic methods from fasting samples obtained yearly during DCCT and on alternate years during EDIC.
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In this study we used conventional enzymatic methods to assay lactate and creatinine.
T cells were isolated from lung parenchyma using either a conventional enzymatic method or by explant culture with Statamatrices as described in Materials & Methods section (4).
Zuppi et al have shown earlier that lactate levels in urine by conventional enzymatic methods are comparable to levels by proton NMR spectroscopy of urine [ 40]and that two methods can be used interchangeably.
Serum lipids, insulin, and glucose were determined by conventional enzymatic methods.
Serum total lipids and glucose were assayed by conventional enzymatic methods on a Hitachi 911 automated analyzer from Roche Diagnostics (Laval, QC, Canada).
A typical Holstein herd using conventional breeding methods will produce 48percentt female offspring and 52percentt male.
DNA was extracted using conventional extraction methods [ 19].
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