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These deletions were undetectable using conventional cytogenetic methods [ 12].
We have analysed four primary NSCLC tumours and two established NSCLC cell lines using modern molecular cytogenetic techniques that allow genetic aberrations to be elucidated, which would have been uninterpretable using conventional cytogenetic methods.
The fosmid clone was tested on metaphase and interphase cells of healthy donors, obtained using conventional cytogenetic methods, to analyze the position and strength of the signal, the presence/absence of background and cross-hybridization and the hybridization efficiency.
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Interpretation of the complex genomes characteristic of solid tumours is also technically difficult using conventional cytogenetic banding methods.
Our results showed that when using conventional cytogenetic techniques only 22.6% of patients had positive results.
Chromosome damage was measured in 4 patients using conventional cytogenetic culture, harvest, and fixation in combination with spectral karyotyping (SKY).
Case 1 and Case 2 were ascertained via routine clinical genetics investigations in a regional genetics service laboratory using conventional cytogenetic analysis of metaphase chromosomes from peripheral blood cells.
The most extensive study of this kind in the dog to date (Hahn et al, 1994) used conventional cytogenetic techniques to identify the single most commonly occurring aberration in chromosome preparations generated from each of 61 canine lymphoma cases.
The conventional cytogenetic methods, 'chromosome banding' and 'karyotyping' are very informative and are still commonly used.
Of these, 35 deletions (78%) and 23 duplications (92%) were not detected by conventional cytogenetic methods.
Disorders now recognized as CGS often have subtle cytogenetic changes that cannot always be resolved with conventional cytogenetic methods.
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