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Analysis of genome organization for the RNase Dre1 and RNase Dre2 genes was done using contigs obtained by combining information from cDNAs cloned by our laboratory (supplementary dataset) and ESTs obtained from NCBI EST-other.
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$Calculated using contigs obtained in common assembly of ESTs from both the libraries.
Additionally, we confirmed that the low detection accuracy of DECs was eliminated when using the subset of contigs (annotated contigs) obtained by applying this method.
The program BWA [ 42] was used to align raw reads against all contigs obtained by Velvet assembly for calculating the coverage of the sequencing.
This design enabled an elevated number (8) of distinct oligos to be used for each of the 48,021 contigs selected from the overall total of 59,000 contigs obtained by assembling the GS FLX sequences.
Similarly, just a simple count of the number of mis-assembled contigs obtained by alignments to the reference genome (if available), a metric typically used to compare short-read assemblies, is not satisfying either, because it does not take into account the various structural properties of the contigs and of the reads contained in it.
The resulting assembled sequences and the remaining singletons were then used as input for a second MIRA run (see methods) of assembly in order to produce meta-contigs from a fraction of partially redundant contigs obtained by the first run.
Contigs obtained by hybridization in this report were compared and verified with those from the genome-wide physical contig map (Xu et al., in preparation).
The contigs for PGB02 (15 contigs) and PGB04 (14 contigs) obtained by automated sequence assembly were manually curated.
The influenza contigs obtained using the above software were reassembled in Sequencher Version 4.8 (Genecodes).
We further compared differentially expressed genes obtained by microarray and contigs obtained in transcriptomic data using Pearson uncentered correlation.
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