Sentence examples for using constructs provided by from inspiring English sources

EF-1a-GFP lentivirus was made using constructs provided by Dr. Charles Murry (University of Washington).

A third-generation self-inactivating lentiviral transfer vector plasmid with a gene encoding firefly luciferase or d2Venus (Nagai et al, 2002) (provided by Dr A Miyawaki, RIKEN, Wako, Japan) under the control of the elongation factor 1- α (EF1 α) promoter was produced using constructs provided by Dr H Miyoshi (RIKEN, Tsukuba, Japan) by standard molecular biological techniques (Miyoshi et al, 1999).

We found that most of the logic of the medical process involved could be readily described using the constructs provided by PROforma.

The pYFPN43-AKINβ construct (provided by A. Ferrando), to express YFPN-AKINβ, was used as a negative control.

The mCherry-AMPKα fusion protein was made using a mCherry construct (provided by R. Tsien, University of California, San Diego, San Diego, CA) at the N terminus fused in-frame to AMPKα into the pUAST vector.

HER2-YFP was constructed using a LTR-2/erbB-2 HER2) LTR-2/erbB-2 HER2ed by Dr LE Samelson, Nconstructsda, MD, USA) as a providedlate and a pcDNA3.1-mYFP construct as a vector (gift from Roger Y Tsien, University of California at San Diego, USA).

Human recombinant XPG was expressed in insect cells using the XPG baculoviral construct (provided by Drs. Joyce Reardon and Aziz Sancar, University of North Carolina) and purified essentially as described [ 26].

We used CHAC1 pcDNA6 plasmid (CHAC1 V5eGFP construct) provided by the University of California at Los Angeles, USA.

Metropolitan patent counts were constructed using data provided by the U.S. Patent and Trademark Office, see Text S1.

The database is constructed using annotations provided by NetAffx [ 18] and the Gene Ontology Consortium [ 19].

A plasmid construct containing the sequence for human accelerated region 1 (HAR1) used by Beniaminov et al. was provided by A. Krol of the Institut de Biologie Moléculaire et Cellulaire.