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In addition, we performed metabolomic experiments using conditioned media from Wnt11 knockdown and control 4T1 cells.
We further investigated keratinocyte action using conditioned media.
The purpose of this study was to examine ethanol's delayed effects (termed hangover) using conditioned place testing.
The present study compared the taste profile of MSG with four amino acids (glycine, l-proline, l-serine and l-arginine) using conditioned taste aversion, detection threshold, and taste discrimination methods.
The results suggest using conditioned food prepared in a single batch and the C, N and P content of the food should be measured.
The gelatinolytic activity of MMP-2 using conditioned media was measured as described previously [30].
To determine the effects of PTEN upregulation in glioma cells, we performed the spheroid migration assay using conditioned media from co-cultured glioma and hUCBSC cells.
The cytokine antibody array was performed using conditioned media to check the possible modulation of various cytokine levels in MMP-2siRNA treatments (data not shown).
The ability to detect changes in samples grown in direct coculture, mixed monoculture (through the use of inserts or related physical barriers), and standard monoculture using conditioned media allow such processes to be attributed to specific levels of interactions.
The Human Cytokine Array 3 kit was used to verify relative levels of cytokines released from 4910 and 5310 cells after pSV and pM treatments using conditioned media following manufacturer's instructions (Ray Biotech®, Norcross, GA) as described previously [44].
The same effect can be observed simply by using conditioned media coculture from MSCs.
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