In this work, the stability, thermodynamics and structure of the functional dimeric Fur A from Anabaena sp. PCC 7119 were studied by using computational methods and different biophysical techniques, namely, circular dichroism, fluorescence, Fourier-transform infrared, and nuclear magnetic resonance spectroscopies.
Although most reports on RrmiRs have mainly concentrated on TEs, there are a few reports on miRNAs derived from tandem array sequences using computational methods and biological experiments [15], [19], [81].
XL participated in the analysis of tasiRNA and natsiRNA using computational methods and generated the miR173-tasiRNAs PPR/ TPR network.
Using computational methods and cross-species comparisons among human, chimpanzee, mouse and cattle, we analyzed promoters of genes encoding factors involved in IRF and NF-κB pathways.
Comparison of our data obtained using computational methods and findings obtained using experimental approaches, based on structural and biochemical characterisation, shows remarkable consistency (Figs. 2A and 3).
Then we investigated the possible genetic determinants of phenotypic differences between these strains using computational methods and integrating classical genomics analysis, such as the identification of shared and specific genes with the prediction of regulons for selected transcription factors that are known to play a role during symbiosis.
During the last 20 years, there has been a strong methodological development of spatial conservation prioritization (SCP), which is a sub-discipline of conservation biology that uses computational methods and decision analysis in the allocation of protection or other conservation actions (Moilanen et al. 2009; Kukkala and Moilanen 2013).
Furthermore, the other two genes (ALD5 and ISU2) have been inferred as Leu3 targets using computational methods combining ChIP and expression analyses.
Using computational methods, including docking and molecular mechanics calculations, we have been able to correctly identify the relative order of catalytic activities associated with these four single point mutants.
In contrast with labor-intensive and time-consuming experimental approaches, prediction of S-nitrosylation sites using computational methods could provide convenience and increased speed.
Members of this family have recently been identified using computational methods in the chimpanzee and horse genomes [ 24, 25].
