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Fingerprint contig BAC maps have been constructed for cattle using clones from the INRA BAC library [ 22] and the CHORI-240 BAC library [ 23].
Suppressors of mps3 ∆ were further verified by complementation of the suppression phenotype using clones from the 2µ tiling library, if available (Jones et al. 2008).
The map was generated using clones from the CHORI-243 sheep BAC library and from a cosmid genomic library from Clontech.
Although both libraries yielded acceptable sequence quality, the pYES2/CT library appeared to yield slightly longer sequences; consequently, the remainder of the sequencing was performed using clones from the pYES2/CT library.
In silico identification of these paralogs was in good accordance with fluorescence in situ hybridization (FISH) experiments using clones from the 8p23.1 DEF locus as probes (Additional files 3 and 4).
The microarray slides were printed by The Norwegian Microarray Consortium at The Norwegian Radium Hospital, using clones from the Unigene Image Consortium 40K set, representing approximately 15,000 human genes.
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To validate whether the genes enriched from the n-butanol-challenged libraries were indeed involved in enhanced n-butanol tolerance, we used clones from the ASKA collection [41], which is an ORFeome library collection for E. coli K-12.
We used clones from the RIKEN Arabidopsis full-length (RAFL) cDNA libraries.
Using clones selected from the SS9 genome sequencing project and from a specific genomic library with short inserts, a microarray covering large part of the SS9 genomic sequence (78.0% on chr1, 69.7% on chr2 and 79.3% on plasmid) was prepared and used to identify variable regions in three P. profundum strains.
To supplement the Hf library, we used clones derived from the MD_Bb library developed by the Clemson University Genomics Institute [ 34].
First, about 21% of the expression clones that we made using donor clones from the C. elegans ORFeome library were found to be defective after our first sequencing step.
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