Sentence examples for using clonal cell lines from inspiring English sources

This brief personal view highlights some of the concerns regarding in vitro research, e.g. using clonal cell lines such as PC12 cells and SH-SY5Y cells, to illustrate that many of these concerns may not be justified.

Our findings are based primarily on clinical specimens, and it is not surprising that in vitro studies using clonal cell lines only capture some aspects.

The following experiments were performed using clonal cell lines with the greatest degree of gene extinction.

Studies using DNA methylation (DNAm) [ 32], which is acquired by the Xi but low to absent on the Xa, do not require clonal populations; however, studies of activity [ 23], or absence of active marks such as RNA polymerase II [ 33] must either use proven clonal cell lines or adjust for the extent of skewing of XCI.

The present study was designed to investigate whether OCP can promote the differentiation lineage from osteoblasts to late osteocytes using a clonal cell line IDG-SW3 compared to commercially available sintered β-tricalcium phosphate (β-TCP) and hydroxyapatite (HA) in a transwell cell culture.

The threshold of detection was analyzed by qPCR using a clonal cell line control (HCT116) which, after transduction with a GFP lentivirus, contained one lentiviral integration per cell (see Additional file 3: Figure S3).

Now, McLaughlin et al. used two mouse clonal cell lines to study the chromatin signature of uninduced and induced cells, and found that it correlates strongly with their gene expression states, suggesting a role of chromatin-based mechanisms in MM cell fate.

To examine the heterogeneity in size we used a clonal cell line, YH16.33, to generate detergent-free lipid rafts.

Using this method, homozygous clonal cell lines can be constructed in 5 6 weeks.

To ensure that these results were not the result of cellular abnormalities introduced during clonal selection, HIV replication in the bulk cell lines that were used to generate the clonal cell lines was analyzed.

In order to investigate whether the methylhistone H3 binding function of the G9a ANK domain is required for mESC differentiation and the accompanying de novo DNA methylation of key pluripotency genes, G9a-null mESCs (knock-out; KO) were used to generate stable clonal cell lines expressing either wild-type (+WT) G9a or G9a containing various ANK domain point mutations mentioned above.