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Anthrax was confirmed using classical microbiological methods and PCR as described in Blackburn et al. [ 17].
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For such studies, improved diagnostic tools are needed to complete the currently used classical microbiological methods.
Additionally, use of classical microbiological methods like "sputum culture status" as a biomarker for TB treatment response has limited usefulness in children and extra-pulmonary cases, where appropriate quality sputum samples are hard to get.
An increasing number of molecular techniques for the diagnosis of fungal infections have been developed in the last few years, due to the growing prevalence of mycoses and the length of time required for diagnosis when classical microbiological methods are used.
A combination of classical microbiological methods, epifluorescence microscopy, SEM, TEM, molecular diagnostics, radioisotope labeling and other techniques made it possible for us to obtain convincing proof of the presence of pro- and eukaryotes in the Antarctic ice sheet.
This new diagnosis test, which requires minimal technical skill efficiently completes the classical microbiological methods.
Classical microbiological methods revealed an equivalent number of culturable bacteria in the small intestine of WT and PD-1 /– PD-1 /
Protein extracts were prepared using classical TCA methods [57].
We then quantified invasion efficiencies using classical invasion assay methods.
Data were analysed using classical twin modeling methods.
The relationship of binary data is difficult to identify using classical methods.
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