Sentence examples for using centrifugal filtration from inspiring English sources
Before each experiment [125I]-albumin was purified from free iodine, using centrifugal filtration.
Fractionation of OECM was done using centrifugal filtration devices.
Work in other ECM-rich tissues such as cartilage has shown improved protein resolution on 2-DE following depletion of high Mr glycans using centrifugal filtration [34], [37], [38], anion exchange chromatography [35] and cetylpyridinium chloride precipitation [36], [39].
The reaction was incubated at RT for 5 h and subsequently purified using centrifugal filtration to yield the completed DOTA- and DFO-modified antibodies.
The dialysates were concentrated and a puffer exchange to 100 mM ammonium bicarbonate buffer was performed by filtration using centrifugal filtration units (molecular weight cutoff 10 kDa, Millipore, Billerica, USA).
The fractions that showed only PTDH by SDS PAGE were pooled and concentrated using centrifugal filtration [Amicon membrane, 30000 molecular weight cutoff (Millipore)], followed by buffer exchange or dialysis to 20 mM MOPS (pH 7.6), 100 mM KCl, and 10% glycerol.
Both DR1 and DM proteins were purified and buffer exchanged into PBS (7 mM Na+/K+ phosphate, 135 mM NaCl, pH 7.4) using centrifugal ultra-filtration (Amicon).
After 3 h, the progress of the reaction was assayed with radio-TLC using an eluent of 50 mm EDTA pH 5.0, and the radiolabeled antibody was purified with centrifugal filtration using centrifugal filter units with a 30 000 molecular weight cutoff (Amicon Ultra 4 Centrifugal Filtration Units, Millipore Corp., Billerica, MA) and phosphate buffered saline (PBS, pH 7.4).
Trastuzumab (purchased commercially as Herceptin, Genentech, San Francisco, CA) was purified using centrifugal filter units with a 30 000 molecular weight cutoff (Amicon Ultra 4 Centrifugal Filtration Units, Millipore Corp., Billerica, MA) and phosphate buffered saline (PBS, pH 7.4) to remove α α-trehalose dihydrate, l-histidine, and polysorbate 20 additives.
The device spins the cartridge, using centrifugal force to isolate sperm cells.
The supernatants were concentrated using Centrifugal Filter Units (Millipore).
