Sentence examples for using cell extracts from from inspiring English sources

Production was scaled up and 68 mg/L of vanillin was recovered from 10 mg/L of FA using cell extracts from 1 mg biomass within 30 min.

In order to investigate the role of the TEV protease, three separate pulldowns were completed using cell extracts from MPC-5 cells stably expressing WT1 +KTS).

ChEC was performed using cell extracts from 100 ml formaldehyde-cross-linked yeast cells as described previously [26] with the following exceptions: Formaldehyde was added to cultures at room temperature for 15 minutes.

Additional pulldowns were performed, using cell extracts from MPC-5 cells stably expressing WT1 +KTS -FF-ZZ, but also using control cell extracts from MPC-5 cells expressing the empty vector plasmid.

These results were confirmed by IB using cell extracts from FM82 and FM55 controls and FM82 and FM55 shRNAs.

Quantification of two-color co-localization for DNA-bound 293T-F and 293T-O TERT complexes was consistent across different spot densities per field (A ) and using cell extracts from independent transfections of 293T cells (B ).

Likewise, immunoprecipitation experiments were performed using cells extracts from wild type W1118 flies as well as from flies overexpressing the unrelated Flag-tagged Drosophila protein REEP (Figure 6A, REP) or from flies overexpressing TBPH F/L150 152 (FL), a variant of TBPH mutated within the RNA binding domain (Figure 6A, FL, and see below).

As positive controls for Slt2p activation, we used cell extracts from gas1Δ cells and from wt cells incubated at 37°C for three hours.

Seamless ligation cloning extract (SLiCE) is a simple and efficient method for DNA assembly that uses cell extracts from the Escherichia coli PPY strain, which expresses the components of the λ prophage Red/ET recombination system.

The treatment is conducted using cells extracted from the patient's own fatty tissue or from umbilical cords, both rich with adult stem cells that can give rise to almost any kind of the human body's more than 200 cell types.

Direct evidence for the effect of miR-34c on cyclin E regulation was provided by using cells extracted from a mouse model overexpressing cyclin E, who develops multiple pulmonary adenocarcinomas, and human LC cell lines transfected with miR-34c resulting in repression of cell proliferation [ 77].