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Antimicrobial susceptibility was determined using breakpoints approved by the CLSI [ 9] with the (US) Food and Drug Administration FDAA) breakpoints used for tigecycline [ 10].
Susceptibility of S. pneumoniae isolates to telithromycin was determined using breakpoints approved by both the CLSI and the US Food and Drug Administration (susceptible ≤ 1 μg/mL; intermediate 2 μg/mL; resistant ≥ 4 μg/mL) [ 26, 27].
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In order to categorise the bacteria as susceptible, intermediate or resistant, the species-related breakpoints approved by the European Committee on Antimicrobial Susceptibility Testing (EUCAST) were used, if available [ 4, 5].
Bacteria were classified as susceptible, intermediate or resistant according to the recommendations and the human-specific clinical breakpoints approved by the French Society for Microbiology.
Interpretive criteria for the in vitro susceptibility testing of tigecycline are based on the breakpoints approved by the FDA or on those published by the European Committee on Antimicrobial Susceptibility Testing.
Antimicrobial resistance was assessed using breakpoints recommended by the Clinical and Laboratory Standard Institute (CLSI) guidelines 2008 (M100-S18).
Susceptibility testing of K. pneumoniae was performed by the agar dilution method, using breakpoints set by the Clinical and Laboratory Standards Institute (5, 6 ).
Definitions of penicillin susceptibility varied; some studies used breakpoints established by the Clinical and Laboratory Standards Institute (CLSI) in 2009 [ 53, 74, 83, 88, 90, 100], whereas others used pre-2009 breakpoints [ 7, 9, 15, 17, 30, 34, 63, 73].
Potential carbapenemase producers are currently screened first by susceptibility testing, using breakpoint values for carbapenems (2, 8 ).
Both uses are approved by the F.D.A.
The animal use was approved by UIC IACUC.
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