Sentence examples for using bp clonase invitrogen from inspiring English sources
The PCR product was amplified using primers OsWRKY11-F and R, and cloned into pDONR221 using BP clonase (Invitrogen, Carlsbad, CA) to make an entry clone.
Purified ORF fragments were transferred to pDNR223 using BP clonase (Invitrogen) and from there to p2T7-177 GTW, pAD and pDB using LR clonase (Invitrogen).
The recombinational cloning was performed using BP clonase (Invitrogen) following the manufacturer's instructions.
The PCR product was recombined with pDONR_P4-P1R using BP clonase (Invitrogen) to generate the 5′ element clone p5E_cntn1b 5kb).
Subsequently, the amiRNA construct was cloned into the pC5300 OE vector using BP clonase (Invitrogen, Darmstadt, Germany).
To create entry clones, cDNAs encoding GFP and microRNA constructs against Olig1 or HHM were transferred from pcDNA6.2-GW/EmGFP-mir into pDONR221 (Invitrogen) using BP clonase (Invitrogen).
To construct the eIF4E mutants, the full-length eIF4E coding sequence was cloned into entry vector pDONR207 by recombination reactions using BP clonase II (Invitrogen).
To generate wild-type and phosphomimic ARR1 overexpressing plants, the ARR1 (ARR1; At3g16857) cDNA was PCR-amplified from an Arabidopsis cDNA library using attB primers, and cloned into pDONR221 using BP clonase enzyme mix (Invitrogen).
Site-specific DNA recombination was used to clone the human 3′UTR PCR amplicons into the Gateway® Entry vector pDONR P2r-P3 (Invitrogen), using BP Clonase II Enzyme Mix kit (Invitrogen, Carlsbad, CA) following the manufacturer's specifications.
pLR159 was created using primers attb1unc17pfor and attb2unc17pbac to PCR amplify a short (3678 bp) version of the unc-17 promoter from genomic DNA and recombining the promoter with pDG15 using BP clonase II.
The complete PCR product was cloned into an entry vector with BP clonase (Invitrogen), subsequently cloned into pDEST42 with LR clonase (Invitrogen) and eventually sequenced in its entirety.
