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We also classified unigenes from our experiment using BLAST to query sequence databases (Table 1).
In many cases several of these could be associated with a common reference sequence using blast to query a reference database.
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Subsequently, we used Blast to query these highly represented k-mers against the NLE whole-genome shotgun sequences deposited in the NCBI Trace Archives (http://www.ncbi.nlm.nih.gov/Traces/home/).nih.gov/Traces/home/
abscessus isolates, we used PCR to amplify their hsp65 genes for Sanger sequencing, and then used BLAST to query the sequences to known Mycobacterium species/subspecies gene sequences stored in a web-accessible database of hsp65 locus sequences [ 44].
Following assembly, we used Blast to query the repeats identified in Step 1 against the contigs generated in Step 2 to identify contigs that include transposition-associated protein-coding sequences.
In order to test the ability to detect highly diverged sequences with aCGH, we used BLAST to query the full genome assemblies of each of the heterologous species with the predicted D. melanogaster probe sequences to provide a measure of sequence divergence for comparison to the array-based measures.
An RBH approach uses BLAST to compare a query sequence against another genome [85], [87].
First round of PSI-BLAST uses BLAST to search for proteins similar to query in a given database.
Intronic sequences were identified using BLAST with query sequences from S288C (c ox1-aI1α, aI2, aI3α, aI5α, aI5β, aI5γ, rnl-I1), YJM789 (cox1-aI3γ, aI4β) and S. paradoxus CBS432 (cox1-aI3β).
Gene functions were assigned according to the best match of the alignments using Blast and BlastP (query coverage ≥50%; E-value: 1e-10) againsthehe NCBI NR protein database.
The genomic sequences obtained were compared using BLAST query with the GenBank.
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