Sentence examples for using blast to determine from inspiring English sources

80 clones, randomly selected from each library, were then sequenced and analysed using BLAST to determine gene redundancy.

The contigs in each bin resulting from the 3D binning were characterized using BLAST to determine the dominant species.

The extent of HGT within and between phylogroups was assessed using BLAST to determine the most similar sequence for all genes gained along terminal branches across the phylogeny (fig. 7).

These identified genetic features were individually assessed using BLAST to determine their origin, location within the genome (plasmid or chromosomal borne) and function based on homology to the highest score BLAST hits.

Forward and reverse primers were developed using Primer Express Software Applied Biosystemss, Carlsbad, CA, USA) according to the manufacturer's instructions and they were compared to the B. cenocepacia J2315 database using BLAST to determine their specificity.

To evaluate the hybridization specificity of the array probes, we used BLAST to determine the similarity between all individual probe sequences and the open reading frames in the S. aureus genomes included on the array.

A second in silico validation is performed by using Primer BLAST to determine whether the primer specifically amplifies the gene of interest.

The sequences of differentially expressed genes identified by the microarray experiments were collected from NCBI GeneBank [ 39] and compared them to known sequences from Cotton Gene Index [ 40] using the Basic Local Alignment Search Tool (BLAST) to determine if there was any significant homology to known gene products.

We then used the Basic Local Alignment Search Tool (BLAST) to determine if the di-arginine motif identified in the bioinformatic screen in the first 25 residues were conserved throughout multiple species within the proteins containing one or more transmembrane domains.

This software uses Primer3 to design the primers and then BLAST to determine specificity.

We used blast interrogation to determine the most conserved orthologs supposed to retain the ancestral function of the known human FGF-interactors.