Exact(5)
Sequences obtained from ITS, EF1-α, bloc, and RPB1 gene regions were compared with the NCBI GenBank accessions, using Blast to confirm identifications of all isolate strains.
Each nrITS sequence was searched in nucleotide data base using BLAST, to confirm its plant origin rather than from a possible fungal contamination of the sample.
Nucleotide sequences were searched against the draft A. caninum genome scaffold sequences [39] (Mitreva, unpublished) using BLAST to confirm the identity of the hookworm genomic fragments.
They were tested in silico using BLAST to confirm the Map specificity.
All sequencing results were compared with those present in the NCBI databases using BLAST to confirm the allelic variants.
Similar(55)
The obtained sequences were searched against the NCBI GenBank database using Basic Local Alignment Search Tool (BLAST) to confirm virus identification.
After de novo assembly, L1, L17 and L71 contigs were blasted to confirm identity using BLASTn (Basic Local Alignment Search Tool from http://blast.ncbi.nlm.nih.gov/Blast.cgi) and ordered against complete C. pecorum E58 genome (accession number: CP002608) with progressive Mauve [ 28] to produce single genome scaffold.
Results of best taxonomic matches to the set of predicted ORFs from Naph2 using Blast confirmed that the majority of these matches are to members of the ∂-proteobacteria, thus placing this genome within the δ-proteobacteria lineage.
The nucleotide sequences were then edited and aligned using Blast program; the sequencing confirmed protozoal species according to the GenBank accession numbers for their nucleotide sequences.
Using Blast search, it was confirmed that the toxin homologs were present in all the pathogenic strains of the bacteria.
The latter ORFs were also checked using BLASTP to confirm that no better functional annotation can be obtained by BLAST.
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