To further analyze morphological differences caused by Sar1 mutants we used biochemical analyses and subcellular fractionation of cells co-transfected with either Sar1H79G and Cx43-FP or Sar1T39N and Cx43-FP.
Many parameters have been examined using biochemical analyses, histological examination of specific organs, hematology and the detection of transgenic DNA.
The detection of small peptides being more difficult using biochemical analyses, BactPepDB is thus expected to assist the experimental discovery of new bioactive peptides.
This N-terminal domain includes two chromodomains as well as an extreme N-terminal region, of which a large portion (residues 1 117) is typically truncated in proteins used for biochemical analyses and which is thus of unknown function.
Here, using the A. socius complex of crickets, our goals were to identify protein "X" using biochemical and genetic analyses, sequence and clone the full length transcript that produces protein "X", assess tissue- and sex-specificity of this transcript, and use RNAi technology to knockdown transcript expression and evaluate the phenotypic effects.
The existence of these physiological processes was further elucidated using biochemical and cytological analyses, as well as genetic manipulation of carotenoid biosynthesis in citrus calli, F. hindsii Swingle, and M. hupehensis calli.
In the present report, we assessed the effects of autophagy stimulation by trehalose in a transgenic mouse model of tauopathy, the human mutant P301S tau mouse, using biochemical and immunohistochemical analyses.
Evidence for such events was first obtained using biochemical and structural analyse of vinculin (Johnson and Craig, 1994) which revealed that vinculin is composed of head, neck and tail domains (Fig. 2) with the interaction between head and tail domains masking the binding sites for vinculin-binding partners (Ziegler et al., 2006).
Using biochemical, mutation, and cellular analyses, we characterized important domains involved in the functionality of a RNA-binding protein in RNA splicing.
Using biochemical and cell biological analyses, we show that Bat3 inactivation sustains the C-terminal phosphorylation and nuclear localization of Smad1, 5, and 8 (Smad1/5/8), thereby enhancing biological responses to BMP treatment.
