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Next, we looked at the effects of UV-inactivated WNV particles on astroglia using astroglial cell lines or primary cultures.
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Further, the in vitro effects of purified S100B protein on neutrophil migration and glutamate uptake were also determined in astroglial cell lines or primary cultures.
PAX6 expression in astroglial cell lines (with regulatory roles in differentiation and proliferation) has recently been established (Sakurai & Osumi, 2008; von Bohlen und Halbach, 2011; Gomez-Lopez et al., 2011).
We used SVG-A cells, a subclone of SVG cells (ATCC no. CRL-8621), an SV40-transformed human astroglial cell line and SV-T2 cells (ATCC no. CCL-163.1, Manassas, VA, USA), a mouse fibroblast cell line transformed with SV40.
We found that neither SVs nor exosomes derived from the LPS-stimulated U87-MG astroglial cell line had any effects on SH-SY5Y cell viability under normal conditions (Fig. S1).
SVG cells [37], a human fetal astroglial cell line chronically infected with JCV (Mad-4 strain) were seeded onto 6 well plates overnight.
We used four cell lines.
All experiments were conducted using the three breast cell lines.
GL15 cells were characterised as an astroglial-like cell line by the study of the cell karyotype and immunohistochemical and cytogenetic demonstration of glial fibrillary acidic protein (GFAP) expression [ 12].
All cell lines used were established immortal tumor cell lines.
cell lines using particle bombardment.
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