Sentence examples for using as template from inspiring English sources

The gRNA End1 (E1) with the U6 promoter was amplified by PCR adding Not1 restriction sequence (primers available in Supplementary Table 1) with the GoTaq polymerase (Promega) using as template the pSpCas9 BB -2A-GFP that already contains the E1 gRNA20.

ECL2 was modeled using as template the ELC2 of human kappa opioid receptor since among crystallized GPCRs it has the most similar sequence length with ggTas2r1, does not enter the binding site and does not influence the binding directly, reducing the potential mistakes as a consequence to incorrect loop modeling.

This process starts using as template an NAAM with opened pores at the aluminium alumina interface (Fig. 3a).

The other probe (P2) was generated by using as template, a 954 bp region of the T-DNA obtained by Nco I digestion (Figure 2d).

The ITS (internal transcribed spacers) regions were amplified by PCR (polymerase chain reaction) using as template the genomic DNA extracted from the sample.

The protein was modeled through Swiss-Model server [37, 38], using as template the sterol carrier protein of Aedes aegypti (PDB: 1PZ4) [39] reported in the RCSB Protein Data Bank.

The gene for the VHH domain of about 400 bp (Figure 2B) was amplified with nested primers PMCF, BACK-1, and BACK-2 using as template the 600 bp PCR fragment.

The lacO probe was labeled by random priming, using as template a ∼300 bp fragment containing LacO repeats.

Molecular docking was performed using as template the 2z7x.pdb structure of the TLR-1/2 in complex with the tri-acylated lipopeptide Pam3CSk4 [15] (Figure 7).

A molecular model of the gp125 monomer was generated using as template a crystal structure of gp120 that includes the V3 region [18].

Positive and negative control experiments were performed by using as template either genomic DNA or DNase-treated RNA in the absence of reverse transcriptase, respectively.