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We tested the tumor cell killing potential of this antibody using a real-time live-dead cell cytotoxicity assay using as targets metastatic melanoma cells recognized by this clone (Figure 7 & Supporting Videos S1 and S2).
This hurdle could be partly overcome by using as targets the proportion of patients treated within set times.
For each culture, cytotoxicity assays were performed by using, as targets, cells pulsed in the presence of specific or control peptides or SSL preparations.
When indicated, assays were performed by using, as targets, HBL cells previously cultured in monolayers in the presence of 0 20 m M lactic acid (Sigma-Aldrich, St Louis, MO, USA) for 3 days.
We then performed our antigen recognition assays by using as targets HBL melanoma cells cultured in 2D in the absence or in the presence of graded concentrations of exogenous lactic acid (Sigma-Aldrich, St Louis, MO, USA).
El-Beshbishy et al. (2013) [ 18] compared the PCR amplification using as targets primers that anneal in the regions of ribosomal DNA (rDNA) of the parasite and also that anneal in the kDNA.
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Unmanned drones were used as targets for 23 surface-to-air missiles launched during the day.
Though littered with scrap used as targets, and subject to regular shelling, the land has become a haven for wildlife.
If used as targets, these could indeed hugely distort admissions procedures.But that's not happening.
Aluminum ovoids were used as targets.
Fe, Co, Ni, and Mo were used as targets for the sputter source.
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