Sentence examples for using as secondary from inspiring English sources

Ultrathin sections were collected on nickel mesh grids and processed for immunogold labelling for GABA as described in ref. [49], using as secondary antibodies goat Fab fragments coupled to 10 nm gold particles (British BioCell International, Cardiff, UK).

After blocking for 1 h at room temperature with PBS containing 3% BSA the cells were incubated overnight with anti-Myc (1∶500) and/or anti- Flag (monoclonal or polyclonal, Sigma, at 1∶200) antibodies, using as secondary antibodies the anti-mouse FITC (Sigma) and anti-rabbit Alexa Fluor 568 (Molecular Probes).

To determine CFTR localization in the lysosomes, macrophages were first stained for CFTR using as secondary antibody Alexa Fluor-594 goat anti-rabbit IgG F ab 2, and then they were labelled with the mouse anti human LAMP-1 antibody (Developmental Studies Hybridoma Bank, University of IOWA, IOWA City, IA) and the Alexa Fluor-488 F(ab)2 goat anti-mouse IgG.

To determine cell proliferation, formalin-fixed, paraffin-embedded breast tumours were stained with an antibody against Ki67 (Novacastra), using as secondary antibody a HRP conjugated anti-rabbit (ImmunoLogic).

The fourth author is Richard Rhodes, who wrote two other works that Mr. VanDeMark used as secondary sources, "The Making of the Atomic Bomb" and "Dark Sun".

Transient temperature gradients were used as secondary variables.

The characteristics of MM including MM severity were used as secondary outcomes.

Alexa Fluor-conjugated Donkey 488/594/647 (1 200, Life Technologies, USA) were used as secondary antibodies.

Annual counts of road traffic crashes and injuries were used as secondary outcomes for road safety.

Biological treatment based on rotating biological contactor (RBC) is used as secondary treatment.

The patients' vital signs over the course of the study period were used as secondary measurements.