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Calibration curves based on quantification of peak areas were performed using aqueous calibrators of known concentrations.
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The identification of three further peaks was achieved by comparison with migration times of aqueous calibrators and spiking of the actual serum samples with stock solution of standard substances.
The identification of seven peaks succeeded, again by comparison with migration times of aqueous calibrators and spiking of the serum samples.
Calibration was performed using 7 calibrator points (charcoal stripped human serum enriched with six increasing levels of H6-25(OH)D2 and H6-25(OH)D3).
Measure the amount of FDG activity (in kBq) in the second syringe using the dose calibrator and record the time of measurement T m,2 (hh:mm:ss).
Calibration of the analyzer is checked quarterly using a commercial calibrator.
Each fraction was measured first using the dose calibrator and again, after decay of the primarily eluted 68Ga, using gamma-ray spectrometry.
The radioactivity of the carcass of the mice was measured using a dosi-calibrator (Amersham, Capintec Inc., New jersey, USA).
All eluates were measured immediately after elution using the dose calibrator and later on by means of gamma-ray spectrometry.
Surface expression of CD148 was quantified using the Platelet Calibrator Kit (Biocytex, Marseille, France), as previously described.
The relative expression of SC markers was measured using the same calibrator, the CD56+ sorted population from CES3, expanded in culture for 4 weeks (Pax7 expression characterized in Supplementary Fig. 1b).
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