Sentence examples for using appropriate volume of from inspiring English sources

a. #Transfect 8 µg of DNA per construct using appropriate volume of transfection reagent.

Before cell staining, red blood cell lysis was performed using appropriate volume of lysis buffer (150 mM NH4Cl; 10 mM KHCO3; 0.1 mM diNaEDTA, pH 7.4).

The brain was used later for homogenization using appropriate volume of PBS/protease inhibitor and guanidine/tris HCl solution to bring final concentration of guanidine to 5 M.

High-resolution electroencephalographic (HREEG) techniques allow estimation of cortical activity based on non-invasive scalp potential measurements, using appropriate models of volume conduction and of neuroelectrical sources.

All antibodies were used in an appropriate volume of 10% rabbit serum (Dako, Glosdrup, Denmark) and PBS (Biomerieux, Marcy l'Etoile, France) to reduce unspecific signal.

All antibodies were used in an appropriate volume of 10% rabbit serum (Dako, Glosdrup, Denmark) and PBS (Biomerieux, Marcy l'Etoile, France) to reduce nonspecific signal.

Volume of essential oils tested was varied (20, 40 or 60 μl) by using appropriate number of sterile discs.

Using appropriate aquifer pore volume, a stable pressure that fairly matched the reservoir pressure was obtained.

To obtain the correct ratio of anticoagulant to blood, 21 mL of the citrate suspension was aseptically removed from a standard 500 mL human blood collection bag prior to use to yield the appropriate volume of anticoagulant for the expected 350 ml of blood.

The fold change of each target gene was then calculated using 2 –(ΔCT sample –ΔCT control) were ΔCT is (CT gene of interest-CT reference gene).> hESCs were maintained within PPP-derived hydrogels to passage 10, harvested using trypsin then resuspended in an appropriate volume of hESC media.

The precipitate was taken in appropriate volume of chloroform and used for thin layer chromatography (TLC) using aluminium sheets coated with silica gel 60 (Merck Life Science, Mumbai, India) and a mobile phase composed of chloroform:methanol:formic acid (85:15:1, v/v) as described by Bell et al. (2003).