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Data were analysed using 'Analyse it' version 2.20 software for Microsoft Excel.
The fluorescence density of Cd was calculated by selecting the root sections and measuring the total Integrated Density using "Analyse and Measure" function of the Image J software (NIH, Bethesda, MD, USA).
And the fluorescence density of Cd was calculated by selecting the root tips sections in the figures and measuring the total Integrated Density using "Analyse and Measure" function of the Image J software (NIH, Bethesda, MD, USA).
However, because it is not clear how best to estimate the maximum slope from monotonic clines, we estimated cline widths by fitting parametric sigmoid clines using Analyse 1.3 [ 66].
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AUC ROCs of potentially predictive parameters were compared using Analyse-it software (Analyse-it Software, Leeds Leeds, UK).
Statistical analysis was done using Analyse-it 2.12 (Analyse-it Software, Leeds Leeds, United Kingdom) and SPSS 11.0 for Windows (SPSS, Chicago, IL, US) A P-value < 0.05 was considered statistically significant.
The results obtained from the comparative study were evaluated using Analyse-it software (Version 1.62; Analyse-it Software, Ltd, Leeds, UK).
Analysis was performed using Analyse-it for Microsoft Excel version 2.20 (Analyse-it Software, Ltd. http://www.analyse-it.com/; 2009).
Data were analyzed using analyses of variance.
Statistics were done using analyses of variance.
Data were entered into a Microsoft Access database and analysed using Analyse-it for Microsoft Excel (version 2.11).
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