Sentence examples for using an shRNA vector from inspiring English sources

Knockdown of GABP alpha using an shRNA vector confirms that this protein is important for the function of both the RIBS and UP sites.

To investigate whether the suppression of GnT-IVa expression in Jar could influence the invasive ability in vivo, we established a stable GnT-IVa knockdown model of choriocarcinoma, using an shRNA vector, and analysed the effects on tumorigenicity in nude mice.

Using a shRNA vector to deplete Cyclin T1, we identified a common set of 54 Cyclin T1-dependent genes (CTDGs) in PMA/ionomycin-activated Jurkat T cells, PMA-treated MM6 cells, and LPS-treated MM6 cells.

As a positive control for TRF2 inhibition, we used an shRNA vector against TRF2 (shTRF2), which could reduce TRF2 protein and mRNA levels by ∼80% (Fig. 2A).

To identify Cyclin T1-dependent genes (CTDGs), we used a shRNA vector to deplete Cyclin T1 and evaluated the effects on global gene expression with DNA microarrays.

In addition to infection with the NKI shRNA library, we also used a shRNA vector targeting p53 as a positive control for colony outgrowth of the BJtsLT cells at 39°C.

Knockdown of c- myb was performed using an shRNA expression vector kindly provided by Dr. Tom Gonda [ 6].

To examine the interactions between these different repair systems, we examined whether toxicity, induced by t-BOOH and KBrO3, differs in BER proficient (Mpg+/+, Nth1+/+) and deficient (Mpg−/−, Nth1−/−) mouse embryonic fibroblasts (MEFs) following Msh2 knockdown of between 79 and 88% using an shRNA expression vector.

Therefore, we assessed functions of E-units in the experimental metastatic model by knockdown of GalNAc4S-6ST using an shRNA-expressing vector in LLC cells.

To interrogate this hypothesis, we produced a stable knock-down of Pgc1α using a lentiviral shRNA vector.

shRNAs targeting mouse Miwi and Caf1 were constructed using a lentiviral shRNA vector pSilencer-H1-LV as recently described, and then cotransfected with the respective target expression vector in 293T cells to test their efficacy of silencing before lentiviral packaging (Supplementary information, Figure S8A and S8B, left).