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Briefly, mice were killed using an overdose of isoflurane; the testicles were exteriorized and carefully separated from attached connective tissue and the cremaster muscle was removed.
Briefly, mice were killed using an overdose of isoflurane; the head was removed and the arteries were carefully separated from neural tissue.
Following the iontophoresis, rabbits were euthanized using an overdose of sodium pentobarbital at 0.5, 1, 2, 3, 4 or 8 h post-iontophoresis.
For morphological examinations, fish were sedated using an overdose of tricaine methanesulphonate (MS-222, Sandoz, Basel, Switzerland) and then immediately preserved in alcohol (70%), formalin (4%) or Bouin's solution [46].
Specimens were sacrificed using an overdose of benzocaine.
On the 8th day, the animals were euthanised using an overdose of carbon dioxide.
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When you take your old dog on its last journey, your vet will use an overdose of hypnotics to put him down.
We collected embryos in cold PBS and then moved them into a filtered flask to administer euthanasia using an overdose inhalation of 70 100% CO2 gas.
At the end of the study the animals were killed using an intravenous overdose of sodium thiopental and T61® (Intervet, Unterschleissheim, Germany).
All animals were housed in different cages according to their groups, were allowed unlimited activity, and were sacrificed 8 weeks after the operation using an intravenous overdose of anesthesia.
Four weeks after surgery, the animals were euthanized using an intravenous overdose of pentobarbital.
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