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A compact and two-dimensional atomic force microscope (AFM) using an orthogonal sample scanner, a calibrated homodyne laser interferometer and a commercial AFM head was developed for use in the nano-metrology field.
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The affected proteins were adequately validated using an orthogonal method and independent sample set.
The experimental calibration set was composed of 16 sample solutions using an orthogonal calibration design for two component mixtures.
Validation studies were performed using serum and urine samples from an independent cohort of SLE patients using an orthogonal method.
A total of twenty-seven valid cases using an orthogonal array, L27 (313) orthogonal experimental design (OED) were selected.
A series of hypothetical profiles were generated using an Orthogonal Main Effects Plan (OMEP) [ 43].
Using an orthogonal main effects plan design, a questionnaire with 18 choice tasks was created.
Patients were planned using an orthogonal film or virtual simulation technique.
Attributes and levels were combined using an orthogonal design to produce a number of discrete hypothetical treatments.
However, an explanatory factor analysis using an orthogonal varimax rotation was also performed to ensure comparability with previous validation studies.
Proteins which showed a change during radiotherapy were selected for validation using an orthogonal antibody-based approach.
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