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However, as long as the iterative method is based on the gradient descent and the variable follows the direction of the gradient using an optimized step size, a convergence towards a local optimum is guaranteed.
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For the analysis of the global NBPF expression pattern, we determined relative gene expression levels using an optimized two-step SYBR Green I RT-PCR assay employing several reference genes [19].
Relative expression levels were determined using an optimized two-step SYBR Green I RT-PCR assay [ 27].
Relative SDHD expression levels were determined using an optimized two-step SYBR Green I RT-PCR assay [ 38] with minor modifications in 31 cell lines, 7 normal control samples (human brain, trachea, lung, heart, breast, kidney, liver) and in laser capture microdissected foetal neuroblast cells [ 39].
To facilitate the construction of target-focused libraries for virtual screening, we developed eSynth, a new fragment-based approach to molecular synthesis that follows simple combinatorial chemistry steps using an optimized, graph-based algorithm.
Using an optimized "combinational IBE" process, surface modified sharp steps were prepared resulting in high yield of low 1/f noise devices when combined with high quality YBCO film.
The relatively hydrophobic character of the peptide facilitated effective extraction using an optimized mixture of organic solvents, and a single subsequent chromatography step (reversed-phase HPLC) produced a homogenous bacteriocin preparation.
It calculates Delaunay diagram by using an optimized incremental algorithm.
DNA was extracted using an optimized in-house method [ 15].
Hybridization was performed using an optimized low-background protocol [ 30].
Compared to most of the studies on potential sources of bias in the manuscript reviewing process published up to now, the present study used an optimized strategy with two independent analysis steps.
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