Sentence examples for using an optimized protocol from inspiring English sources
The results we obtained suggest that, by using an optimized protocol, an efficient phage antibody library can be generated.
For analysis, PCR amplification products were mixed with the MapMarker size standard (50 1200 bp, Bioventures Inc., Murfreesboro, TN, USA) and separated by capillary electrophoresis (GenomeLab GeXP Genetic Analysis System; AB Sciex Germany GmbH, Darmstadt, Germany) using an optimized protocol for long DNA fragments as recommended by the manufacturer.
Platelets from whole blood were isolated using an optimized protocol using centrifugal separation in an acid-citrate-dextrose (ACD) buffer, which uses citrate as an anticoagulant.
Hybridization was performed using an optimized protocol for the Lucidea SlidePro hybridization station (Amersham).
These results suggested that by using an optimized protocol and very high repertoire diversity, a compact and efficient phage antibody library can be generated.
Peptide was synthesized on a low loading Rink Amide MBHA resin at a 0.15 mM scale using a Focus XC automated solid phase peptide synthesizer (Aapptec, Louisville, KY) by using an optimized protocol reported previously.
By using an optimized production protocol, VLP titers were increased 2.4-fold obtaining 2.8 μg of Gag-GFP/mL or 2.7 × 109 VLPs/mL according to ELISA and nanoparticle tracking quantification analyses, respectively.
T1-weighted anatomical scans were acquired (MPRAGE, 1 mm3 resolution) and analyzed using an optimized VBM protocol to detect white and grey matter volume reduction without spatial a priori.
In order to analye the subcellular compartmentalization of the plant metabolome, NAF was performed on three independent replicates of pooled Arabidopsis leaves from soil grown plants harvested three hours after the onset of light using an optimized NAF protocol [22] (Text S1).
RNA was purified using an optimized TRIzol protocol in conjunction with the Microarray Tissue Kit (Qiagen; Valencia, CA, USA).
Routinely we obtain 60 80% of cells expressing fluorescent chimeras using an optimized transfection protocol described earlier (Majoul et al. 2006).
