To determine whether raloxifene is a ligand of the AhR, we first performed molecular docking studies with raloxifene using an optimized homology model of the human AhR ligand-binding pocket.
It calculates Delaunay diagram by using an optimized incremental algorithm.
Hybridization was performed using an optimized low-background protocol [ 30].
DNA was extracted using an optimized in-house method [ 15].
We, therefore, used an optimized computation method.
Recently, however, she's switched to using a mobile-optimized Google Sheet to save the list.
We developed a robust macrocyclization method using ring-closing metathesis and synthesized a 45,000-compound library of hybrid macrocycles (named rapafucins) using optimized FKBP-binding domains.
Whole-cell lysates were immunoblotted (IB) using anti-HIF-1α, HIF-2α, HIF-1β, and β-actin antibodies.
Because HIF-1 binding to HREs is methylation sensitive (Wenger et al., 1998), we tested the effect of hydroxymethylation on HIF-1 binding within the HRE using an electrophoretic mobility shift assay (EMSA).
As a first step we examined for spatial association between each class of transcript and HIF-binding sites, using well validated, previously published HIF-1-and HIF-2-binding sites in MCF-7 cells [ 14].
