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The average oil droplet diameters for all five oil droplet types were obtained by measuring ten different oil droplets of each type in a single field using an optical micrometer (data not shown).
Total leaf count and trichome density (the average of two counts made using an optical micrometer and dissecting microscope on each of the midvein and blade) were also recorded at year 1; trichome density has been variously associated with resistance to insect herbivory and with susceptibility to pathogens (Lake and Wade 2009; Monier and Lindow 2004).
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T-cell phenotypes in the placental sections were assessed manually using an ocular micrometer fitted into the eyepiece of the microscope (Olympus Optical Co. Ltd ., and data were calculated as number of cells per 100‐μm field area.
The measurements were taken using an ocular micrometer (accuracy 0.01 mm) of an Olympus SZX9 stereomicroscope.
Colony sizes were measured using an ocular micrometer.
The comet tail length was measured using an ocular micrometer.
Oocyte diameter was measured using an ocular micrometer calibrated with a stage micrometer fitted in a light microscope (Labex, India).
The measurements were taken under 20× magnification using an ocular micrometer.
For routine histological examination, the length of duodenal villi was assessed using an ocular micrometer.
The diameter of the beads was determined using an optical microscope and digital micrometer, and the average values were taken for at least 25 beads.
To investigate surface roughness on the micrometer level, three discs of each surface were investigated at ten sites using an optical interferometer (MicroXamTM, PhaseShift, Tucson, USA).
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