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A band corresponding to each protein was visualized using an Odyssey scanner and Odyssey 2.1 software (Li-Cor, Lincoln, NE).
Wet gels were first scanned for fluorescence using an Odyssey scanner (LiCor Biosciences Nebraska USA) at 780/800 nm and then dried and exposed to a phosphor imager plate overnight.
All western blots were visualized using an Odyssey scanner (Li-Cor Biosciences, Lincoln, NB).
Finally, bound streptavidin was detected using an odyssey scanner (LI-COR, Lincoln, NE, USA), and the images were analyzed with the RayBio analysis tool.
The gels were run at 15 V/cm in 0.5× TBE at 4 °C for 1 h, and scanned using an Odyssey scanner (LI-COR Biosciences).
The nitrocellulose membranes were incubated with IRDye 680CW goat-anti-mouse or IRDye 800CW goat-anti-rabbit secondary antibodies (1 : 10 000) for 90 min at room temperature and visualized using an Odyssey scanner (LI-COR Biosciences, Lincoln, NE, USA).
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We performed western blot analysis for the BCR-ABL1 fusion protein using mouse monoclonal antibody against c-Abl [c-Abl(24-11); Santa CA]z, Santa Cruz, CA] or GAPDH (6C1, Millipore, Billerica, MA) and IR Dye800CW-labelled anti-mouse IgG (Li-cor, Lincoln, NE) and detected the proteins using a Odyssey scanner (Li-cor).
Blots were imaged using an Odyssey infrared scanner (Licor Biosciences).
Quantitative immunoblot analysis was performed using an Odyssey infrared scanner and software (LiCOR).
Western blots were analyzed by densitometry using an Odyssey infrared scanner (LiCor).
Blots were imaged using an Odyssey infrared scanner (Li-Cor Biosciences).
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