Exact(9)
Bands were visualized using an Odyssey imaging system (Li-COR biosciences, Lincoln, NE).
Blots were analyzed using an Odyssey imaging system.
In some experiments, the 'active' conformation of the G protein was stabilized by replacing GDP in the binding and wash buffers with 30 µM GTPγS or a mixture of 30 µM GDP/30 µM AlCl3/10 mM NaF. Immunoblot quantification was performed by infrared imaging following the manufacturer's protocols using an Odyssey imaging system (Li-Cor Biosciences).
For analysis of these levels and those of LC3-II levels in PC12 cells treated with rilmenidine, western blots were probed with secondary antibodies conjugated to IRDye® for detection at 780 or 680 nm (Li-Cor Biosciences) and visualized and quantified using an Odyssey imaging system (Li-Cor Biosciences).
Arrays were imaged using an Odyssey imaging system (LI-COR, Lincoln, NE).
Gels were de-stained with 10% acetic acid (v/v), 1% glycerol (v/v) de-stain solution, and scanned using an Odyssey imaging system (Licor Biosciences, UK).
Similar(51)
Signals were imaged using an Odyssey infrared imaging system (Licor Biosciences).
The images were acquired using an Odyssey infrared imaging system (LI-COR, USA).
The precipitates were analyzed by Western blot with the indicated antibodies and visualized by incubation with IRDye800-conjugated secondary antibodies (diluted 1∶10,000) using an Odyssey infrared imaging system (LICOR Inc., Germany).
Blots were visualized by using an Odyssey infrared imaging system (Li-Cor, Inc).
Quantification was performed using an Odyssey infrared imaging system (Li-Cor Biosciences).
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