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Labelled cells per millimeter were determined using an ocular grid.
Then, superficial vital dye staining of C. pyrrhogaster embryos was performed at the distance of 1.3 mm from the dorsal lip using an ocular micrometer in 0.1× Steinberg's solution.
Colony sizes were measured using an ocular micrometer.
The comet tail length was measured using an ocular micrometer.
Sperm viability was assessed using an Eosin dye.
Mix the dye using a dye brush until the two are evenly blended.
When using a chemical dye, apply a fixative based on the type of fabric being dyed.
Or two parts conditioner one part dye if you're using a light dye!
If you are using a liquid dye, adjusting the water-to-dye ratio can create softer (add more water) or more vivid colors (add more dye).
Using a clean dye brush, mix the dark hair dye and developer in a disposable plastic bowl until evenly blended.
Similar results were obtained using a green fluorescent dye for MA- cells and a red dye for MP cells (dye swap experiment, data not shown).
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