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We measured each eye's axial length, anterior chamber depth, and anterior corneal curvature using an ocular biometer (IOLMaster, Carl Zeiss Meditec, Dublin, CA) and incorporated these measurements into a model eye to convert the lateral field size in our images from angular (i.e., degrees) to physical (i.e., micrometers) units [ 18].
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Labelled cells per millimeter were determined using an ocular grid.
The measurements were taken using an ocular micrometer (accuracy 0.01 mm) of an Olympus SZX9 stereomicroscope.
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The comet tail length was measured using an ocular micrometer.
Histomorphometry was performed using an ocular mounted 10 × 10 graticule at a magnification of 100×.
Cell counting was performed using an ocular grid at × 200 magnification.
For routine histological examination, the length of duodenal villi was assessed using an ocular micrometer.
The measurements were taken under 20× magnification using an ocular micrometer.
The prosoma length and width of all individuals were measured using an ocular micrometer on a stereomicroscope (Olympus SZ61).
Histomorphometry was performed using an ocular-mounted 10 × 10 graticule at a magnification of × 100.
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