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As shown in Figure 1, an object image is usually magnified by using an objective lens in the DHM system, and the enlarged object beam is reflected from front and back surfaces of an optical window glass.
The rigid endoscope was positioned orthogonally to the sample plane at a distance of 2cm and the sample plane was imaged onto a CCD Retiga-2000RR, QInc.ing, InCanadanada) using an objective lens (SM1NR05, Thorlabs, Inc., US).
In transmission mode (Fig. 3a), a laser beam is focused onto the sample through a transparent substrate using an objective lens with high numerical aperture (NA), which is usually >1.0 [29].
It has a power of about 20 mW and is focused down to the diffraction limit using an objective lens with a magnification of ×50 and a 0.75 numerical aperture.
Using an objective lens of, e.g., 40× magnification, the spot size is about 50 µm in diameter for the 785 nm laser, whereas for the 535 nm laser the analyzed area is reduced to 35 µm in diameter.
For this analysis TPF images were acquired using an objective lens with 1.3 NA.
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For lung tissue, we typically use an objective lens setting of 20X to inspect our IF-IHC stained sections during laser capture; the field images are clear and identifying different cell types is relatively easy (Figs. 6- 8).
To form a multi-trap rotating tweezers with our propelling beams, we use an objective lens (60X, NA = 0.85) with relatively low magnification so to match the size of individual traps with the typical size of the particles used in our experiments.
An X-ray Fresnel zone plate (ATN/FZP-SiC86/416, NTT Advanced Technology Corporation [NTT-AT], Tokyo, Japan) was used as an objective lens with a focal length of 261 mm and an outer-most zone width of 86 nm.
Cells were randomly sampled using a sampling grid of squares 75 μm x 75 μm in dimension, separated by 150 μm in x and y directions, using StereoInvestigator software using a x20 objective lens.
IRBA custom software is prompted to automatically focus the laser beam onto the sample using an aspheric objective lens with f/0.5 numerical aperture and a 20× magnification.
More suggestions(15)
using an objective test
using an ordinary lens
using an analytical lens
using an axiological lens
using an compound lens
using an optical lens
using an objective index
using an 40x lens
using an objective approach
using an intersectional lens
using an objective set
using an narrow lens
using an cylindrical lens
using an objective cross-correlation
using an 24mm lens
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