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The osteoclast, osteoblast and osteoid surfaces were measured using an objective eyepiece Leitz integrateplatte II.
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The mineralizing surfaces were measured in the same area using the objective eyepiece Leitz integrateplatte II.
Counting was done on five squares at a total magnification of 400×, using a 10× eyepiece and a 40× objective.
Whole tumour sections were scanned using an eyepiece graticule marked with 25 random points (Chalkley grid) and a ×20 objective.
The BGBBs and the EECCBs in the vascular regions supplied by the PA were counted on semithin toluidine-blue stained sections at a final magnification of ×1000, using a 10× eyepiece and a 100× oil objective (Fig. 8A).
The number of blood vessels was counted per 0.22 mm using a microscope eyepiece graticule (×10 ocular and ×20 objective).
Wound area was measured using an eyepiece graticule and calibrated using a haemocytometer grid, at 10× magnification.
Size was measured using an eyepiece graticule.
Crown-rump length was measured using an eyepiece graticule.
In each field, we counted at least 300 cells using an eyepiece graticule to prevent recounting.
Colony areas were measured using an eyepiece graticule after calibration with a slide graticule.
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