Quantification was done by using a morphometric grid.
The area of meninges was determined using a morphometric grid as described in detail before (Dal-Bianco et al., 2008).
Counting of neurons, oligodendrocytes, dystrophic axons and TUNEL-positive cells was performed in cortical layers III to V. Quantitative analysis was done using a morphometric grid within the ocular lens counting 10 microscopic fields of 0.0576 mm per region of interest (normal appearing grey matter and lesion).
Cell densities (neurons per cubic millimeter) were estimated in 8 μm Neu-N-stained slices at 400x magnification with a morphometric grid methodology using Abercrombie's correction [ 36] as previously described and well established in the literature for surgical hippocampal fragments [ 1, 3, 8, 11, 37– 41].
Cell densities (neurons per cubic millimeter) were estimated in 8 μm Neu-N stained slices at 400x magnification with a morphometric grid methodology using Abercrombie's correction [ 50] as previously described and well established in the literature for surgical hippocampal fragments [ 3, 5– 7, 51– 51].
Cell densities (neurons per cubic millimeter) were estimated in 8-µm Neu-N stained slices at 400× magnification with a morphometric grid methodology using Abercrombie∼s correction (Abercrombie, 1946) as previously described (Babb et al., 1984a; Mathern et al., 1995a; Kandratavicius et al., 2012).
Power calculations have confirmed this hypothesis, especially if CAN is evaluated using a morphometric technique.
TSR was measured using a morphometric approach (point counting) on digitised histologic hysterectomy specimens.
Sections were overlaid with a morphometric grid and the area of myelin loss and loss of astrocytic antigens was manually determined by counting the grid points over the respective areas.
For quantification, the immunostained sections were overlaid by a morphometric grid and inflammatory cell numbers or acutely injured or transected axons were manually counted in 5 100 fields per tissue area.
Labelled cells per millimeter were determined using an ocular grid.
