Sentence examples for using an isotype control from inspiring English sources
A second set of tubes was also set using an isotype control primary mouse monoclonal IgM antibody (Cat no. sc-3881, Santa Cruz).
Specific staining was confirmed using an isotype control antibody.
Specificity of epitope staining was proofed using an isotype control (black line).
Staining for the isotype controls was performed simultaneously using an isotype control antibody (BD PharMingen).
By using an isotype control capture antibody, non-specific signal due to RF can be identified [ 20, 21].
No background staining was seen using an isotype control antibody on tissue from all patients, before and after treatment (data not shown).
Control experiments using an isotype IgG control antibody did not significantly affect the migration speed of either cell type.
Flow cytometry analysis using an isotype matched control antibody did not show up regulation of MyD88 (data not shown).
The area of positivity was determined using an isotype matched control Ab. 104 events for each sample were acquired.
Negative controls were performed either by omitting the primary antibodies or using an isotype-matched control.
Positive cells were estimated using an isotype-matched control negative Ab at an equivalent concentration.
