Sentence examples for using an isotype IgG from inspiring English sources

Control experiments using an isotype IgG control antibody did not significantly affect the migration speed of either cell type.

Omitting the primary Ab or using an isotype-matched IgG (Fig.  2A, a3, a4) show no ECs staining; a light-reddish background caused by the NovaRed substrate revealed thinner alveolar walls and evident signs of damage under siRNAITSN conditions (Fig.  2A, a4).

Tumor-bearing mice were treated with trastuzumab or scIgG-T (with a compromised Fc) weekly at 5 mg/kg for four weeks and an isotype IgG was used as nontreatment control.

An isotype IgG antibody was used as negative control and specific Tia1 immunoprecipitation was checked by western blot.

An isotype control was conducted using a mouse IgG isotype control serum (Dako Corp., Carpinteria, CA).

As expected, exposure to LPS significantly increased Pol-II occupancy at the promoter regions of MHC-II), CIITA), NOS2),TNF-α), and IL-6), whereas minimal to no Pol-II occupancy was observed using the isotype IgG antibodies (data not shown).

Further, ChIP analysis performed using isotype IgG antibody on the same samples display a very low reactivity.

As negative control, ChIP analysis using isotype IgG antibody was performed on the same samples.

Negative controls included omitting the primary antibodies and using isotype IgG instead of the primary antibodies for each parameter.

On the other hand, controls using isotype IgG did not show any effect (data not shown), suggesting that the specific antibodies interacted with target protein functionality.

In order to verify the staining specificity, parallel blocking experiments involving preabsorption of the specific primary antibody with blocking peptide or using a primary isotype-matched irrelevant IgG were performed.