Sentence examples for using an isocratic solvent from inspiring English sources

The reversed-phase HPLC-UV was used for the ametryn determination, using an isocratic solvent delivery system (acetonitrile: H2O, 60 40), flow-rate of 0.8 mL min−1 and a UV wavelength of 220 nm.

The dried material obtained from plant extraction was subjected to reversed-phase HPLC using an isocratic solvent system consisting of 55% HCl 0.01 M, 25% acetonitrile, 19% methanol, and 1% water.

Ten milliliter fractions were collected separately, and the final purification of compound was accomplished via HPLC using an isocratic aqueous acetonitrile solvent system.

The peptide without biotin was synthesized for further conjugation using Rink Amide AM resin using the same method and purified by C18 reversed-phase HPLC (Proto 300, 150 × 20 mm; flow rate, 7 mL/min) using an isocratic method (95% of solvent A); in 30 min >95% pure peptide was obtained: Him-Suc-GSYK m/ z [M]+ 645.3, found m/ z [M + H]+ 646.8, [M + 2H]2+ 323.4.

Soluble cation content (Rb+) was determined as described in [ 27] using an isocratic method with 20 mM metanosulphonic acid solution.

The protein digests (5 µL) were injected into a reversed-phase C18 PepMap trapping column (0.3×5 mm, 5 µm particle size, Dionex) equilibrated with 0.1% TFA/2% acetonitrile (v/v) and washed for 5 min with the equilibration solvent at a flow rate of 25 µL/min, using an isocratic loading pump operated through an autosampler.

equilibrated with 0.1% formic acid/2% acetonitrile v/v and washed for 5 min with the equilibration solvent at a flow rate of 25 µl/min, using an isocratic loading pump operated through an auto-sampler.

The protein digests (5 μL) were injected into a reverse-phase C18 PepMap trapping column (0.3 × 5 mm, 5-μm particle size, Dionex Inc ., equilibrated with 0.1% formic acid/2% acetonitrile (v/v) and washed for 5 minutes with the equilibration solvent at a flow rate of 25 μL/minute using an isocratic loading pump operated through an auto-sampler.

Chiral separation was optimized on a CHIRALPAK® ID column using an isocratic mobile phase of acetonitrile/water (60 40, v/v).

The quinalphos residue analysis was conducted using an isocratic mobile phase of methanol.

Trifluralin detection was carried out using an isocratic mode with acetonitrile and water (75:25 % v/v).