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The analysis was performed on a reversed phase C18 column using an isocratic mixture of trifluoroacetic acid 0.15% in water and methanol 80 20 as mobile phase.
The separation was performed on a Zorbax XDB-C18 column (4.6 × 150 mm internal diameter, 5-μm particle size; Agilent Technologies, USA) using an isocratic mixture of acetonitrile: water (50 50, v/v) as the mobile phase at a constant flow rate of 1 ml/min.
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Chiral separation was optimized on a CHIRALPAK® ID column using an isocratic mobile phase of acetonitrile/water (60 40, v/v).
The quinalphos residue analysis was conducted using an isocratic mobile phase of methanol.
Trifluralin detection was carried out using an isocratic mode with acetonitrile and water (75:25 % v/v).
The mobile phase consisted of 25%% (A) ultra-pure water and 75%% (B) methanol using an isocratic elution.
Total mannan and glucan were determined by HPLC using an isocratic method and a Refractive Index (RI) Detector.
Crotonic acid was determined using a high-performance liquid chromatography system (Thermo-Fischer Scientific, USA) with a Nucleosil C8 column (Macherey Nagel, Germany) using an isocratic method.
The 300 μL of supernatant was concentrated and then analyzed by RP-18 HPLC using acetonitrile/water/formic acid (60 40 0.1) using an isocratic mode.
Samples were analyzed by using an isocratic HPLC method described previously with minor modifications [92].
GSNO analytes were eluted using an isocratic method of 95% formic acid (0.1%) in water and 5% methanol.
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