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The cells were regularly monitored using an inverted light microscope, and the culture medium was changed every 2 days.
These cells were evaluated daily using an inverted light microscope, and based on the cell density and the amount of mineral precipitation in this latter culture, we decided to terminate the experiments with the cells grown on the disks.
Immunohistochemistry was analyzed using an inverted light microscope (Olympus, Center Valley, PA).
Previously, MODS plates have been visualized only using an inverted light microscope (in our laboratory the NIKON Eclipse TS100-F with an infinity correction optical system).
Cells were maintained in a 37°C tissue culture incubator and after 2 weeks (6 weeks for cells from xenografts) all colonies ≥20 cells were counted in duplicate or triplicate wells using an inverted light microscope.
Wells were subsequently photographed using an inverted light microscope at 20x magnification and tube formation analyzed.
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Utilizing the fluorescence of EGFP expressed in the cells, we were able to demonstrate distribution of cells in a oligo poly ethylene glycol) fumarate) hydrogel material and on a titanium fiber mesh scaffold using an inverted fluorescent light microscope.
Tubule counts were determined in 10 randomly selected fields per well using an inverted Leica DMIL light microscope (Leica Microsystems GmbH, Wetzlar, Germany) at 100x magnification as described previously [ 34].
The effects on lymphatic tube formation including the capillary-like structures, the total number of cell clusters and branching of tube formation (i.e., capillary-tube number) of each group were observed using an inverted phase-contrast light microscope (Olympus IX70) as described previously [ 23].
Cell proliferation in wounded monolayers was visualized by light microscopy using an inverted Olympus CKX41 microscope.
Cell morphologies of treated and untreated cells were analyzed by phase contrast light microscopy using an inverted Axiovert 200 microscope.
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