AMPA-R/NMDA-R ratio was assessed in voltage-clamp mode using an internal solution containing (in mM) 130 CsCl, 4 NaCl, 2 MgCl2, 1.1 EGTA, 5 HEPES, 2 Na2ATP, 5 sodium creatine phosphate, 0.6 NandTP, and 0.1 spermine.
These experiments were performed using an internal solution containing 10 mM HEPES.
Recordings were made from dopamine neurons that expressed D2S or D2L receptors using an internal solution containing EGTA (0.1 mM).
Whole-cell patch clamp recordings were made from SNc dopamine neurons using an internal solution containing the calcium chelator, BAPTA (10 mM), as used previously (Neve et al., 2013).
FS cells were recorded using the above internal solution, while pyramidal cells were recorded using an internal solution with the KCl concentration raised to 15 mM and K-methylsulfate lowered to 117 mM to depolarize the reversal potential of Cl− (−52.5 mV).
Whole-cell recordings were carried out at −60 mV using an internal solution containing 105 mM CH3O3SCs, 10 mM CsCl, 15 mM CsF, 4 mM MgCl2, 5 mM EGTA, 0.25 mM CaCl2, 10 mM HEPES and 4 mM Na2ATP, adjusted to pH 7.2 using CsOH.
For current-clamp recordings, we used an internal solution of K-Gluconate (in mM), 120; KCl, 20; HEPEs, 10; EGTA, 1.1; MgCl2, 2; CaCl2, 0.1, pH 7.2, and external solution of NaCl (in mM), 140; KCl, 2; MaCl2, 6; HEPEs, 5, pH 7.2.
Samples were prepared volumetrically using an internal standard solution in 2% nitric acid.
Urinary samples were diluted about 20-fold using an internal standard solution.
However, using recording electrodes with an internal solution with high Cl− concentration reduced the intensity of TH co-labeling, in some cases to background (recording duration 16.7±0.9 min; n = 10).
No run-down was observed in whole-cell patch-clamp experiments using a "minimal" internal solution without ATP.
