Sentence examples for using an integration vector from inspiring English sources

To enhance pradimicin production, ACCase from Streptomyces coelicolor and two regulatory genes metK1-sp and afsR-sp from Streptomyces peuticus were overexpressed into A. hibisca using an integration vector pSET152 under the control of the strong ermE* promoter.

To identify the gene in the SCCmec region that is responsible for suppressing the colony spreading of S. aureus, we integrated various flanking regions of the mecA gene of the SCCmec region (Figure 2A) into the chromosome of the Newman strain, a laboratory MSSA strain, using an integration vector by a single homologous recombination to examine the colony spreading of the recombinant strains.

The mcherry fluorescent reporter strains were constructed using an integration vector for insertion of alleles between lctP and aspC on the gonococcal chromosome, following a previously reported approach (Mehr and Seifert, 1997).

Here, we sought to reprogram NB cell lines using an integration-free Sendai virus vector system.

Plasmids used are in Table 2. To generate an integration vector to express GFP fused to Pep12 from the PEP12 gene locus, pBS GFP-PEP12:: natNT2 was made.

To circumvent this problem, an integration vector pBGE [18] was used to successfully clone only the ribosomal binding site (RBS) and the coding sequence of the levT gene, creating plasmid pBGE-TNP.

Srivastava et al. co-bombarded a cre gene construct and an integration vector into maize callus containing the a target construct for site-specific integration [ 72].

We complimented this mutant through chromosomal integration of MtbDGC in ΔMSDGC-1 strain using pMV361 integration vector containing Rv1354c gene.

The general procedure for using the integration vector is illustrated in Figure 3.

OppA-KO was complemented with a single copy of His-oppAMTB or its mutants by integration into the chromosome of M. smegmatis using the integrating vector pOPP206 harboring the His-oppAMTB gene under the control of the hsp60 promoter.

We previously have demonstrated that this yeast is a unique host for recombinant expression of human CYP11B2 (aldosterone synthase), and here we report the functional production of human CYP11B1 (steroid 11β-hydroxylase) in S. pombe using our new integration vector pCAD1.