We set out to investigate the mechanisms of high light acclimation in Phaeodactylum tricornutum using an integrated approach involving global transcriptional profiling, metabolite profiling and variable fluorescence technique.
For V-ATPase activity, the V-ATPase-driven pumping of hydrogen ions into lysosomes was measured by acridine orange fluorescence quenching (excitation at 495 nm; emission at 530 nm) using an integrated spectrofluorometer (Photon Technology International, Birmingham, NJ, USA).
The cells were observed using a fluorescence microscope (Axio Observer Z1, Carl Zeiss MicroImaging, Inc., Welwyn Garden City, UK), and photographed using an integrated camera with the appropriate filter for detection.
We analysed data using an integrated approach.
Images were obtained using an integrated digital camera.
This dynamic range could be greatly increased by using multiple fluorescence measurements to compute an integrated fluorescence value, but we did not do this in these experiments because our purpose was to examine single time-point measurements for monitoring large numbers of samples.
Dear Sir: With interest, we have read the article by Roberto Rey-Dios and Aaron A. Cohen-Gadol: Technical principles and neurosurgical applications of fluorescein fluorescence using a microscope-integrated fluorescence module (Acta Neurochir. [2013] 155:701–706).
Integrated fluorescence values were divided by the corresponding ROI area to yield an integrated fluorescence density for each stereocilia tip.
We used an integrated qualitative and quantitative methods design.
MiaPaCa-2 cells, which were cocultured with NIH-3 T3 cells with/without FAP expression, were examined for cell cycle distribution by using the integrated fluorescence intensity which reflected the cellular DNA content (Fig. 3a).
