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Four microliters of sample was injected into the GC-MS in splitless mode, using an injection time of 1 min, with the injection temperature set at 280°C.
As a compromise between the four responses, the optimum condition was obtained in 18 mM ammonium acetate in methanol (MeOH):acetonitrile (ACN):glacial acetic acid (66 33 1%, v/v/v) using an injection time of 4 s, the voltage and the temperature of 28 kV and 24 °C, respectively.
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Mass spectra were also collected using flow-injection analysis of fractions of the HPLC eluate, using an injection cycle time of 0.8 min.
In this research, a pyramidal kagome structure, which is known as one of the most effective light weighting structures, was developed and fabricated with polypropylene using an injection molding process to reduce fabrication time and cost.
The results shown in Figure 3 were obtained using a trap injection time of 3.24 ms and ejection pulse of 480 μs.
Fragment analysis was carried out by electrophoresis on an ABI3130 × l 36 cm capillary using POP-7 polymer with an injection time of 30 s at 1 kV.
All samples were denatured at 96°C for 3 min and then placed on ice prior to electrophoresis on an ABI 3100 36 cm capillary, using POP-4 polymer with an injection time of 45 s.
For all PRTs fragment analysis of the test and reference loci was carried out by electrophoresis on an ABI3100 36 cm capillary using POP-4 polymer with an injection time of 30 s at 1 kV.
Pooled sample were heat denatured in formamide (HiDi, Applied Biosystems) for 2 minutes at 94°C and subsequently snap frozen before being run using a 5 second injection time using a Rox GS-500 size standard (Applied Biosystems).
A measured 0.1-0.3 0.1-0.3yte volume injection was given using a timed pressure injection facility of a Pneumatic PicoPump (World Precision Instruments, Stevenage, UK).
This delay was determined by assessing the time to parenchymal enhancement on power injection DSA, using an identical injection rate and catheter position (Fig. 2).
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