Sentence examples for using an initial cell from inspiring English sources

In contrast to BeWo cells, HPECs are contact-inhibited and formed a confluent monolayer after 3 d when using an initial cell number of 1.0 × 105 cells (Fig. 2a,c).

Cells were inoculated in a 250 mL Erlenmeyer flask containing 100 mL liquid TAP using an initial cell concentration of 5 × 104 cells mL−1, and these were kept in the dark for 96 h.

Experiments were performed in 96-well plates using an initial cell density of 1000 cells per well in a final volume of 200 μL with 10% v/v Alamarblue (Serotec).

All the algae tests were conducted using an initial cell density of 1.0 × 10 cells/mL at 20 ± 1°C with continuous shaking at 100 rpm and under an illumination of 4,000~6,000 lux.

A common and simple procedure now used in our laboratory is to enrich a tissue preparation for precursor cells using an initial low-speed separation in 20 35% Percoll (empirically determined for each strain and species).

Total PBMC RNA was extracted with the RNeasy extraction kit on the 'QIAcube' extraction device (Qiagen Hombrechtikon, Switzerland) using an initial volume of 0.35 ml for cell lysis, DNAse I digestion on the extraction column and a final elution volume of 0.1 ml.

Cells grown at pH 5.0 for 8 h in chemically-defined medium containing either 0 or 6 g L−l acetic acid were used to inoculate, at an initial cell concentration of 1 g (cell dry weight) L−1, anaerobic bioreactors containing chemically-defined medium at pH 3.7 with 6 g L−1 acetic acid.

The TCPS groups can only be comparable in view of the hMSCS behaviour using different initial cell density (i.e., 15 6250 cells/cm2 vs 4690 cells/cm2).

In this study, we demonstrated that high viabilities of short and long-term cancer 3D cultures could be achieved in our 3D μFCA using different initial cell seeding densities (i.e., 10 and 60 million/mL).

In tests with C. reinhardtii, approximately one in every ~ 3 × 107 initial cells contained an edited version of the targeted FKB12 gene (i.e., an average of ~ 3 colonies with an edited FKB12 gene per electroporation using 108 initial cells).

MOI of 2.5 was used for initial cell death analyses as shown in Fig. 2.Under this adenoviral transducing condition, greater than 90% infection efficiency was achieved with these viral stocks (data not shown).