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Specifically, we chose infrared dyes with non-overlapping infrared emission spectra (Alexa-fluor 680 and Alexa-fluor 750) to distinguish total and phospho-Bsk (p-Bsk) on the Western blot using an infrared imager.
The detection of proteins was performed using an infrared imager (Li-Cor, Inc., Lincoln, NE, USA).
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Twenty-four hours after i.v. injection, animals were imaged using an infrared fluorescence imager [ 41], revealing a strong and selective antibody accumulation of SIP L19) and SIP(G11) in the lesions present in the arthritic limb.
The equipment includes an infrared imager for following the temporal evolution of the temperature in the rear face of the tested sample.
Coomassie Blue-stained gels were scanned using an Odyssey Infrared Imager (LI-COR Biosciences) and images were despeckled using ImageJ (http://rsbweb.nih.gov/ij/).
Proteins were detected using an Odyssey infrared imager (Licor, UK) and densitometry performed using ImageJ.
Primary antibodies were detected using LI-COR IRDye Infrared Dye secondary antibodies and visualized using an Odyssey Infrared Imager (LI-COR Biotechnology).
A missile launch is observed using an infrared detector.
Skin temperature was monitored using an infrared thermometer.
Consider the advantages of using an infrared (IR) security camera.
For analysis, cells were washed with PBS, fixed using 4% paraformaldehyde, permeabilized using 0.1% Triton X-100 in PBS, blocked, and then incubated with Anti-phospho-S6K antibodies in LI-COR blocking reagent overnight and average florescence intensity (in arbitrary units) of each well was quantitated using ODYSSEY infrared imager (LI-COR Biotechnologies).
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