Sentence examples for using an immunoaffinity column from inspiring English sources

Analytical methods for OTA detection/identification are generally based on liquid liquid extraction, clean-up using an immunoaffinity column (IAC), and identification by reversed-phase high pressure liquid chromatography with fluorescence detection (HPLC-FLD).

The targeted high abundance proteins, including albumin, IgG, antitrypsin, IgA, transferrin, and haptoglobin in the samples were depleted by using an immunoaffinity column (Agilent Technologies, USA).

The anti-human PSA IgE was purified using an immunoaffinity column containing anti-human IgE (omalizumab, Xolair®, Genentech, Inc. San Francisco, CA) coupled to cyanogen bromide-activated Sepharose (GE Healthcare, Piscataway, NJ) as recommended by the manufacturer.

Remnant like particle cholesterol (RLP-C) level was measured using an immunoaffinity mixed gel containing anti-apolipoprotein (apo) A-I and anti-apo B monoclonal antibodies.

The first biomarker research on the trichothecene deoxynivalenol (DON, vomitoxin) was initiated in 2003 when Meky et al. [ 13] developed an LC-MS-based assay to measure the sum of free DON and DON glucuronides (DON-GlcAs) combined after enzymatic hydrolysis and use of an immunoaffinity column (IAC) as a sum parameter in human and rat urine.

The method uses an acidified solvent extraction, an immunoaffinity column (IAC) for clean-up, liquid chromatography-tandem mass spectrometry (LC-MS/MS) for identification and quantification, and a uniformly stable isotope-labelled OTA (U-[C20]-OTA) as an internal recovery standard.

The retained material eluted with 60% methanol was concentrated by using an evaporator and loaded onto an immunoaffinity column.

The sample extract (10 ml) was diluted to 100 ml with water and cleaned-up by an immunoaffinity column (IAC) (VICAM AflaTest™ WB, Waters, Milford, MA, USA).

Spin labeling to introduce the nitroxide side chain designated R1 and purification of each mutant were conducted on an immunoaffinity column as described previously.

An immunoaffinity column was generated using covalent coupling of the G7 anti-human CLU monoclonal IgG1 to cyanogen bromide (CNBr -activated Sepharose 4B resin (Sigma, St. Louis, MO).

We analyzed maize samples for aflatoxin levels at the National Public Health Laboratory in Nairobi, Kenya, using the immunoaffinity column (AflaTEST; VICAM, Milford, MA, USA) method 977.16 (Association of Official Analytical Chemists 2005) that was provided by VICAM.